Information-enriched characterization of cell populations. Morphological facts from photos can insert to your richness of flow cytometry information. For example, the determine shows an if not typical apoptosis assay utilizing Annexin V and PI, including mobile imaging to characterize cells in Every population to expose morphologically distinct options. These insights could not are attained from multiplex staining by yourself.
Regardless of whether you’re a starter or a professional, check out our assets to advance your investigation and know-how in flow cytometry.
(B) Uncooked 264.seven macrophages are sometimes annotated inaccurately with respect to particle rely due to their complexity and noncircular borders. The quantity of singlets readily available for Assessment will increase once the consumer trains and employs a far more exact design.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing having a higher-velocity brightfield digicam for simultaneous substantial throughput flow cytometry and large resolution brightfield imaging.
"An image isn't really very well worth one thousand dots, but it surely actually helps to see pictures. It is great to determine pictures that correlate with staining. Such as, AnnexinV-constructive cells which are morphologically unique than wholesome cells, or CD14+ monocytes which can be larger sized and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Moreover, it can be illuminating to check out the amount of doublets slip by means of common singlet gates, and fantastic to confirm the status of unpredicted double-favourable activities in my Evaluation."
Pair your Attune flow cytometer using an autosampler for improved workflow efficiency, and include robotic integration for max throughput.
Constant impression quality even at substantial throughput Acoustic concentrating in addition to a substantial-pace digital camera Blend to image these T cells constantly at minimal or significant flow fees. Easily modify focus and digicam settings to fulfill experimental needs.
The Attune NxT cytometer is definitely the reputable workhorse instrument your lab can depend upon for dependable and reliable overall performance. The Attune CytPix product includes a substantial-speed brightfield camera and AI-pushed graphic Evaluation application, with current upgrades such as machine learning applications and an intuitive consumer interface, enabling Improved details precision, precision, and more rapidly insights into sample biology.
End users can view function visuals in actual-time to substantiate sample high quality, enhance protocols, and document the morphology of each and every cell population discovered for analysis. Imaging seize also benefits from acoustic concentrating, which really helps to situation the cells to ensure a pointy, centered impression is attained.
AI-driven automatic image Evaluation With all the Attune Cytometric Software interprets occasion attributes into unique morphology parameters that could be combined with typical fluorescence and scatter parameters to expose extremely reproducible populace modifications and unique subsets.
We Formerly demonstrated the power of imaging Auto-T/Ramos mobile interactions. Allow’s check out just the populace of finest curiosity, the double favourable situations, to learn more. We can now use prolonged morphological parameters (listed here, circularity and skewness of intensity) to even more look at the characteristics of such populations and refine gating on these situations, rising facts robustness.
Leverage the strength of advanced website technology and innovation to accelerate your discoveries. Have faith in during the Attune Flow Cytometer loved ones to provide the dependability, velocity, and accuracy that established your get the job done apart.
Adaptable payment plan from Agilent Money Options let you have the vital instrumentation you would like for quick undertaking, or to mature your lab.
At the guts of every Attune Flow Cytometer lies our innovative acoustic concentrating technological innovation, which makes use of seem waves to meticulously align cells within the flow mobile. This modern approach surpasses classic hydrodynamic strategies, causing faster processing speeds, Excellent data excellent, and amplified sample throughput.
The Attune Xenith cytometer is a superior-parameter spectral analyzer, furnishing both equally spectral unmixing and traditional flow capabilities for in depth and flexible cell Examination.
The Attune CytPix Flow Cytometer permits buyers to visualize samples during acquisition, verify the accuracy of gating methods, and assist effects with reputable documentation for publication needs.
What appeared to be a homogeneous population is really extra various—an Perception that should be considered when interpreting effects.